abstract：OBJECTIVE:Granulocyte colony-stimulating factor (G-CSF) is used to boost granulocyte counts in immunocompromised patients, but its effects on the immune system may be counterproductive. We tested the hypothesis that G-CSF-mobilized peripheral blood stem cell (PBSC) products are immunologically downregulated based on ge...

journal_title：Experimental hematology

authors： Buzzeo MP,Yang J,Casella G,Reddy V

更新日期：2007-09-01 00:00:00

abstract：:We compared the cell division behavior of CD34(-) and CD34(+) (CD33/CD38/CD71)-negative (Lin(-)) CD133(+) cord blood cells stimulated with the cytokines Flt3-ligand, stem cell factor, and thrombopoietin. Within a 4-day time frame, Lin(-)CD34(-) CD133(+) (CD34(-)) cells underwent more cell divisions in serum-free cultu...

journal_title：Experimental hematology

authors： Götze KS,Schiemann M,Marz S,Jacobs VR,Debus G,Peschel C,Oostendorp RA

更新日期：2007-09-01 00:00:00

abstract：OBJECTIVE:We previously showed enhanced engraftment of human T cells in the transgenic NonObese Diabetic/severe combined immunodeficient (NOD/scid)-DR1 mice, compared to NOD/scid mice. We now characterize their immunobiology, innate immunity, and intrahepatic neonatal engraftment of cord blood mononuclear cells (CBMNC)...

journal_title：Experimental hematology

authors： Camacho RE,Wnek R,Fischer P,Shah K,Zaller DM,Woods A,La Monica N,Aurisicchio L,Fitzgerald-Bocarsly P,Koo GC

更新日期：2007-08-01 00:00:00

abstract：OBJECTIVE:Preservation of hematopoietic stem and progenitor cells from early radiation-induced apoptosis is the rationale for emergency antiapoptotic cytokine therapy (EACK) after radiation accidents. This strategy is based on the combination of stem cell factor + Flt3-ligand + thrombopoietin + interleukin 3 (SFT3). Th...

journal_title：Experimental hematology

authors： Hérodin F,Roy L,Grenier N,Delaunay C,Baugé S,Vaurijoux A,Grégoire E,Martin C,Alonso A,Mayol JF,Drouet M

更新日期：2007-08-01 00:00:00

abstract：OBJECTIVE:MicroRNAs (miRNAs) are an abundant class of small noncoding RNAs that regulate diverse cellular functions by sequence-specific inhibition of gene expression. We determined miRNA expression profile during erythroid differentiation and putative roles in erythroid differentiation. METHODS:The expression profile...

journal_title：Experimental hematology

authors： Zhan M,Miller CP,Papayannopoulou T,Stamatoyannopoulos G,Song CZ

更新日期：2007-07-01 00:00:00

abstract：OBJECTIVE:Endothelial progenitor cells (EPCs) are used for angiogenic therapies or as biomarkers to assess cardiovascular disease risk. However, there is no uniform definition of an EPC, which confounds EPC studies. EPCs are widely described as cells that coexpress the cell-surface antigens CD34, AC133, and vascular en...

journal_title：Experimental hematology

authors： Case J,Mead LE,Bessler WK,Prater D,White HA,Saadatzadeh MR,Bhavsar JR,Yoder MC,Haneline LS,Ingram DA

更新日期：2007-07-01 00:00:00

abstract：OBJECTIVE:Donor T cells expressing lymph node homing receptors are the foremost initiators of acute graft-vs-host disease (aGVHD), and a high proportion of CD4(+)CCR7(+) T cells in human leukocyte antigen-matched allografts has been shown to confer a high risk of aGVHD without interfering in other outcomes. METHODS:Na...

journal_title：Experimental hematology

authors： Yakoub-Agha I,Saule P,Depil S,Grutzmacher C,Boulanger F,Magro L,Jouet JP,Dessaint JP,Labalette M

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVE:Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to specifically stimulate proliferation of CD34(+) hematopoietic progenitor cells. Although signal transducers and activators of transcription 3 (STAT3) is believed essential for transduction of GM-CSF-induced cell proliferation, the sig...

journal_title：Experimental hematology

authors： Gu L,Chiang KY,Zhu N,Findley HW,Zhou M

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVE:This article evaluates patients with essential thrombocythemia (ET) to determine whether the V617F mutation in the JAK2 gene affects platelet hemostatic and adhesive molecules, platelet-polymorphonuclear leukocyte (PMN) interactions, and PMN-activation characteristics, as well as plasma hypercoagulation marke...

journal_title：Experimental hematology

authors： Falanga A,Marchetti M,Vignoli A,Balducci D,Russo L,Guerini V,Barbui T

更新日期：2007-05-01 00:00:00

abstract：OBJECTIVE:Strategies to expand hematopoietic stem cells (HSCs) ex vivo are of key interest. The objective of this study was to resolve if ability of HOXB4, previously documented to induce a significant expansion of HSCs in culture, may extend to other HOX genes and also to further analyze the HOX sequence requirements ...

journal_title：Experimental hematology

authors： Ohta H,Sekulovic S,Bakovic S,Eaves CJ,Pineault N,Gasparetto M,Smith C,Sauvageau G,Humphries RK

更新日期：2007-05-01 00:00:00

abstract：OBJECTIVE:Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is expressed by the malignant cells of about 30% of cases of Hodgkin's lymphoma (HL) and is therefore a potential target for immune attack. Given the predominantly immunosuppressive nature of HL infiltrating lymphocytes (HLILs) and the ability ...

journal_title：Experimental hematology

authors： Marshall NA,Culligan DJ,Tighe J,Johnston PW,Barker RN,Vickers MA

更新日期：2007-04-01 00:00:00

abstract：OBJECTIVE:Normal tissues, including the central nervous system, tolerate single exposures to narrow planes of synchrotron-generated x-rays (microplanar beams; microbeams) up to several hundred Gy. The repairs apparently involve the microvasculature and the glial system. We evaluate a hypothesis on the involvement of by...

journal_title：Experimental hematology

authors： Dilmanian FA,Qu Y,Feinendegen LE,Peña LA,Bacarian T,Henn FA,Kalef-Ezra J,Liu S,Zhong Z,McDonald JW

更新日期：2007-04-01 00:00:00

abstract：:Chronic myeloid leukemia (CML) is caused by Bcr-Abl, a constitutively active tyrosine kinase that is the result of a reciprocal translocation between chromosomes 9 and 22 and cytogenetically evident as the Philadelphia chromosome. Imatinib (Glivec, Gleevec), a specific small molecule inhibitor of Bcr-Abl, has become t...

journal_title：Experimental hematology

authors： Deininger MW

更新日期：2007-04-01 00:00:00

abstract：OBJECTIVE:The tumor suppressor p15Ink4b (Ink4b) is a cell-cycle inhibitor that is inactivated in a high percentage of acute myeloid leukemia and myeloid dysplasia syndrome cases. Despite this, the role of Ink4b in hematopoiesis remains unclear. Here we examined the role of Ink4b in blood cell formation using Ink4b-defi...

journal_title：Experimental hematology

authors： Rosu-Myles M,Taylor BJ,Wolff L

更新日期：2007-03-01 00:00:00

abstract：OBJECTIVE:To assess the contribution of bone marrow (BM)-derived endothelial progenitor cells (EPCs) to the neovascularisation of cutaneous incisional wounds. METHODS:Lethally irradiated C57Bl/6 mice were transplanted with BM mononuclear cells from Tie2/lacZ mice, which constitutively overexpressed beta-galactosidase ...

journal_title：Experimental hematology

authors： Bluff JE,Ferguson MW,O'Kane S,Ireland G

更新日期：2007-03-01 00:00:00

abstract：OBJECTIVE:The evolutionarily conserved Kit receptor is vital for function of hematopoietic stem cells (HSC). Kit(W-41) (W-41) and Kit(W-42) (W-42) are single residue changes in the KIT intracellular phosphotransferase domain, while Kit(W-v) (W-v) is a single residue change in the ATP binding domain. This study tests ho...

journal_title：Experimental hematology

authors： Sharma Y,Astle CM,Harrison DE

更新日期：2007-02-01 00:00:00

abstract：OBJECTIVE:The significant role of direct contact between hematopoietic progenitor cells (HPC) and the cellular microenvironment for maintaining "stemness" has been demonstrated. Human mesenchymal stem cell (MSC) feeder layers represent a surrogate model for this interaction. Specific adhesion molecules are responsible ...

journal_title：Experimental hematology

authors： Wagner W,Wein F,Roderburg C,Saffrich R,Faber A,Krause U,Schubert M,Benes V,Eckstein V,Maul H,Ho AD

更新日期：2007-02-01 00:00:00

abstract：OBJECTIVE:To determine the response of bone marrow progenitor cells from patients with myelodysplastic syndromes (MDS) to culture in physiologic oxygen tension. METHODS:Methylcellulose progenitor assays using both unfractionated bone marrow mononuclear cells (MNCs) and purified CD34(+) progenitors were performed in at...

journal_title：Experimental hematology

authors： Thompson JE,Conlon JP,Yang X,Sanchez PV,Carroll M

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVE:Recent clinical trials of adoptive immunotherapy showed diminished reactivity of human T cells upon ex vivo manipulation. For a safe and effective clinical application of human T cells, it is necessary to improve ex vivo manipulation procedures and evaluate their impact on in vivo functionality. However, ther...

journal_title：Experimental hematology

authors： van Rijn RS,Simonetti ER,Hagenbeek A,Bonyhadi M,Storm G,Martens AC,Ebeling SB

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVE:The Hedgehog family of intercellular proteins has a crucial role in embryonic development. Recent experimental data suggests that the Hedgehog pathway may play a role in early hematopoiesis and angiogenesis. Stem cell leukemia (SCL), a basic helix-loop-helix (bHLH) transcription factor, is essential for the s...

journal_title：Experimental hematology

authors： Hochman E,Kinston S,Harmelin A,Göttgens B,Izraeli S

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVE:The OP9-DL1 culture system is an in vitro model for T-cell development in which activation of the Notch pathway by Delta-like 1 promotes differentiation of mature T cells from progenitors. The roles of specific cytokines in this culture system have not been well defined, and controversy regarding the role of ...

journal_title：Experimental hematology

authors： Wang H,Pierce LJ,Spangrude GJ

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVE:The hematopoietic system is nurtured by a supportive stroma environment allowing maintenance and differentiation of hematopoietic stem cells (HSC). However, only a limited number of these stromal cell clones support hematopoiesis in the absence of cytokine supplementation. So far, only two bone marrow-derived...

journal_title：Experimental hematology

authors： Weisel KC,Gao Y,Shieh JH,Moore MA

更新日期：2006-11-01 00:00:00

abstract：OBJECTIVE:Generation and expansion of pluripotent unrestricted somatic stem cells (USSCs) and mesenchymal cells (MSCs) from human cord blood as well their functional characterization were assessed. METHODS:USSC generation was initiated from fresh and cryopreserved cord blood (CB). Culture conditions and functional ass...

journal_title：Experimental hematology

authors： Kögler G,Sensken S,Wernet P

更新日期：2006-11-01 00:00:00

abstract：OBJECTIVE:Resveratrol (3,4',5,-trihydroxystilbene, RV), an ingredient of wine, is an inhibitor of the proliferation-linked enzyme ribonucleotide reductase (RR) and shows a broad spectrum of cytotoxic effects against human cancer cells. In order to enhance these effects, we introduced additional hydroxyl moieties into t...

journal_title：Experimental hematology

authors： Horvath Z,Murias M,Saiko P,Erker T,Handler N,Madlener S,Jaeger W,Grusch M,Fritzer-Szekeres M,Krupitza G,Szekeres T

更新日期：2006-10-01 00:00:00

abstract：OBJECTIVE:Adenoviral vectors (Ad) were widely used in gene therapy and study of gene function, but the commonly used serotype 5 adenovirus-based vectors (Ad5) could poorly transduce hematopoietic cells because of low expression of viral receptors on these cells. To overcome this limitation, we developed a retargeting a...

journal_title：Experimental hematology

authors： Lu ZZ,Ni F,Hu ZB,Wang L,Wang H,Zhang QW,Huang WR,Wu CT,Wang LS

更新日期：2006-09-01 00:00:00

abstract：OBJECTIVE:Graft-versus-leukemia effects of donor lymphocytes have been considered to be central to the therapeutic benefit of nonmyeloablative allogeneic hematopoietic cell transplantation (HCT) for malignant diseases. Surprisingly, some patients who reject donor grafts following nonmyeloablative HCT have sustained rem...

journal_title：Experimental hematology

authors： Saito TI,Rubio MT,Sykes M

更新日期：2006-09-01 00:00:00

abstract：OBJECTIVE:We have previously shown that Flt3 ligand (FL)/Flt3 signaling regulates hematopoietic cell migration by modulating the SDF1alpha/CXCR4 signaling pathway. Herein, we evaluated whether a functional interaction between SDF1alpha/CXCR4 signaling and internal tandem duplication (ITD) of Flt3 regulates aberrant hem...

journal_title：Experimental hematology

authors： Fukuda S,Pelus LM

更新日期：2006-08-01 00:00:00

abstract：OBJECTIVE:Recruitment of endothelial progenitor cells to the sites of ischemia has recently been suggested as a mechanism of tissue repair. Here we address the hypothesis that the hypoxia-inducible full-length endothelial-monocyte-activating polypeptide II (EMAP II) provides a mechanism to recruit late outgrowth highly...

journal_title：Experimental hematology

authors： Hou Y,Plett PA,Ingram DA,Rajashekhar G,Orschell CM,Yoder MC,March KL,Clauss M

更新日期：2006-08-01 00:00:00

abstract：OBJECTIVE:Hematopoietic stem cells (enriched in fraction of CD34+ cells) have the ability to regenerate hematopoiesis in all of its lineages, and this potential is clinically used in transplanting bone marrow or peripheral blood stem cells. Our objective was to assemble a suitable method for evaluating gene expression ...

journal_title：Experimental hematology

authors： Koutna I,Klabusay M,Kohutova V,Krontorad P,Svoboda Z,Kozubek M,Mayer J

更新日期：2006-07-01 00:00:00

abstract：OBJECTIVE:To study the early time course of engraftment of human mesenchymal stem cells in fetal sheep heart and determine the relative roles of proliferation and homing in formation of aggregates of human Purkinje fiber cells. METHODS:The human sheep xenograft model was utilized for these studies. Prior to injection ...

journal_title：Experimental hematology

authors： Colletti EJ,Almeida-Porada G,Chamberlain J,Zanjani ED,Airey JA

更新日期：2006-07-01 00:00:00

abstract：BACKGROUND:In patients treated with rituximab and alemtuzumab for lymphomas or CLL, antibody-dependent cellular cytotoxicity (ADCC) is a major mechanism of action. Therefore, assessment of ADCC is mandatory to understand the complex mechanisms leading to the anti-lymphoma effects of monoclonal antibodies (mAb). Due to ...

journal_title：Experimental hematology

authors： Fischer L,Penack O,Gentilini C,Nogai A,Muessig A,Thiel E,Uharek L

更新日期：2006-06-01 00:00:00

abstract：OBJECTIVE:Monocyte-derived dendritic cells (DCs) play important roles in the immune response against infections and malignancies. Human herpesvirus 6 (HHV-6) infects monocytes and is reactivated in immunodeficient patients. To clarify the mechanisms of HHV-6-induced immunodeficiency, we investigated the effect of HHV-6...

journal_title：Experimental hematology

authors： Niiya H,Lei J,Guo Y,Azuma T,Yakushijin Y,Sakai I,Hato T,Tohyama M,Hashimoto K,Yasukawa M

更新日期：2006-05-01 00:00:00

abstract：OBJECTIVE:Many clinical gene therapy trials have described poor engraftment of retrovirally transduced CD34(+) cells. Because engraftment is dependent upon successful homing of graft cells to the bone marrow (BM), we examined whether retroviral-mediated gene transfer (RMGT) induces a homing defect in CD34(+) cells. ME...

journal_title：Experimental hematology

authors： Hall KM,Horvath TL,Abonour R,Cornetta K,Srour EF

更新日期：2006-04-01 00:00:00

abstract：OBJECTIVE:Mesenchymal stem cells (MSC) raise high hopes in clinical applications. However, the lack of common standards and a precise definition of MSC preparations remains a major obstacle in research and application of MSC. Whereas surface antigen markers have failed to precisely define this population, a combination...

journal_title：Experimental hematology

authors： Wagner W,Feldmann RE Jr,Seckinger A,Maurer MH,Wein F,Blake J,Krause U,Kalenka A,Bürgers HF,Saffrich R,Wuchter P,Kuschinsky W,Ho AD

更新日期：2006-04-01 00:00:00

abstract：OBJECTIVE:Several compounds, including butyrate and trichostatin A, have been shown to activate gamma-gene expression via p38 mitogen-activated protein kinase (MAPK) signaling. In eukaryotic cells, reactive oxygen species (ROS) act as signaling molecules to mediate phosphorylation of tyrosine kinases such as p38 MAPK t...

journal_title：Experimental hematology

authors： Hsiao CH,Li W,Lou TF,Baliga BS,Pace BS

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVE:In utero hematopoietic cell transplantation (IUHCT) is a non-ablative approach that achieves mixed allogeneic chimerism and donor-specific tolerance. However, clinical application of IUHCT has been limited by minimal engraftment. We have previously demonstrated in the murine model that low-level allogeneic ch...

journal_title：Experimental hematology

authors： Ashizuka S,Peranteau WH,Hayashi S,Flake AW

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVE:Using transplantation of a clonal population of cells derived from a single hematopoietic stem cell (HSC) of transgenic enhanced green fluorescent protein (EGFP) mice, we have documented the hematopoietic origin of myofibroblasts, such as kidney mesangial cells and brain microglial cells. Because myofibroblas...

journal_title：Experimental hematology

authors： Ebihara Y,Masuya M,Larue AC,Fleming PA,Visconti RP,Minamiguchi H,Drake CJ,Ogawa M

更新日期：2006-02-01 00:00:00

abstract：OBJECTIVE:Tumor-associated antigens (TAA) are the basis for antigen-specific immunotherapy. The human homolog of the murine double-minute 2 oncoprotein (MDM2) is a putative TAA because it is overexpressed in several malignancies, including chronic lymphocytic leukemia (CLL) cells compared with normal B lymphocytes. PA...

journal_title：Experimental hematology

authors： Mayr C,Bund D,Schlee M,Bamberger M,Kofler DM,Hallek M,Wendtner CM

更新日期：2006-01-01 00:00:00

abstract：OBJECTIVE:It has been shown previously that infection with diverse viruses induces alterations in bone marrow lineage-specific progenitor cells. As complications arising from secondary bacterial infections can adversely affect the host, we investigated whether virally induced hematological alterations could contribute ...

journal_title：Experimental hematology

authors： Hyland L,Villarreal-Ramos B,Clarke B,Baaten B,Hou S

更新日期：2005-12-01 00:00:00

abstract：OBJECTIVE:Recognition of E- and P-selectins on vascular endothelium by their leukocyte glycoprotein counterreceptor P-selectin glycoprotein ligand-1 (PSGL-1) initiates and sustains leukocyte rolling, culminating in extravasation of lymphocytes from blood into organs. PSGL-1 is rendered functional by terminal glycosylat...

journal_title：Experimental hematology

authors： Eom HS,Rubio MT,Means TK,Luster AD,Sykes M

更新日期：2005-12-01 00:00:00